DNA storage and testsfor CsW's

[mijke]

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Thanks in advance!

Possibility of international DNA storage and tests for CsW's

Currently, only a few DNA tests for genetic diseases in dogs of the CsW breed are available. Research is ongoing and we expect a lot more tests in the near future.
For example several laboratory's are working on DNA tests for PRA, Epilepsy, Cancer, HD in different dog breeds.

The owner/breeder of a dog can choose, which test he would like to have performed in his dog.

Until now, a breeder/owner had to submit a separate sample from his dog (EDTA blood or swab) for each individual test.

Recently, the possibility to store DNA isolated from dogs can be utilized to run a number of different genetic tests, even after some time has passed after submittal of the sample.

Every owner can send 2 ml EDTA blood of his CsW to the storage lab.
The lab will isolate and purify DNA, which can then be used to run an unlimited number of tests now and in the future! (in every lab an owner wishes)

How an owner can store EDTA blood of his CsW:

• The dog owner can go to his vet with a blood sample form and a copy of the pedigree of his CsW.
• A blood sample form of Laboklin is to download HERE (as pdf)
• The veterinarian collects 2 ml EDTA blood, checks the chip and signs the blood sample form.

Blood and form are then send to:
LABOKLIN GmbH und Co. KG
Steubenstraße 4
97688 Bad Kissingen
Deutschland

After the lab has received the blood they will send an owner number and the bill with info to the owner.

The price:

• DNA storage for 25 years can be offered for 33 Euro per EDTA blood sample. (Special offer for all CsW owners!)
• This service includes a double DNA isolation as well as storage of the EDTA-blood sample.

How it works:

• DNA is isolated twice from EDTA blood
• The two isolated DNA samples will be stored at different locations for security reasons
• Each DNA isolation results in approximately 100µl.
• To run a genetic test, a volume of 5µl - 20 µl is necessary
• That means that about 20 DNA tests can be performed from the stored DNA isolation.
• If the number of tests is exceed, new DNA has to be isolated.
• The costs for a such a new DNA-isolation are 16.50 (2 caps)
• Additionally, Laboklin in Germany also stores EDTA blood (approx. 2 ml).
• Therefore the number of DNA tests that can be performed is almost unlimited!

Conditions:

• The owner remains the owner of the blood and DNA isolation of a dog
• It is possible to just store EDTA blood and the DNA isolation without obligation for a test in this lab.
• The owner can decide which tests he wants to make.
• There are no additional costs for using DNA destillation to perform a test (besides the normal test price)
• Laboklin can send the DNA isolation of a CsW to a lab the owner chooses.
• If the owner wants Laboklin to send the DNA-isolation to another lab, they only have to pay handling and shipping (depending on the country, weight, ...)

When an owner wants to perform a test of the stored DNA:

• When the EDTA blood is stored at Laboklin laboratory, the owner receives an owner number.
• When the owner wants to perform a test of the stored DNA isolation, he can send a request for the test with his owner number to Laboklin.
• By email to [email protected] or by mail to LABOKLIN, Steubenstr. 4, 97688 Bad Kissingen, GERMANY or by fax to +49-971-7202995
• When this test is available at Laboklin lab the owner only has to mention the name of the test
• When the test is only available in another lab, the owner has to provide the exact name of the test and the complete address and info of the lab!
• After a test is made, the owner receives the test result and bill from the lab that performed the test.

The price for a test:

• Every test has a standard price in a lab, so it depends on the test.
• There are no additional costs for sending DNA isolations for tests to Laboklin.
• If an owner wants Laboklin to send the DNA-isolation to another lab, they only have to pay handling and shipping. (depending on the country, weight, ...).

Currently Laboklin has these tests available for CsW's:

• DM (Degenerative Myelopathy) Price: 66 Euro
• MH (Maligne Hyperthermia) Price: 50 Euro

Laboklin also can send DNA for the Dwarfismtest to the uni of Utrecht! Price 110 Euro (incl. shipping)

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I hope we can make a start now with a kind of international DNA data base for CsW's, when we all store on the same place, while all owners remain owner of the DNA

And maybe then it is possible in future to make on this site a list of all CsW's with DNA storage?

When in future there are interesting test (for example PRA or epilepsy) or a lab needs blood for validation of a test for CsW, we can write about this on the forum.
And every individual owner can decide to cooperate or not.